Study on the effect of sevoflurane on the cognitive function of aged rats based on the activation of cortical microglia

Postoperative cognitive dysfunction (POCD) is a common clinical manifestation that severe complication characterized by decreased learning ability and deterioration of memory following anesthesia surgery. However, the precise mechanisms POCD are not completely understood. Rats were divided into blank control group (Con, n = 12) sevoflurane (Sev, 12). Morris water maze test was performed to evaluate in two groups rats; immunohistochemical staining used detect expression ion calcium-binding adaptor molecule-1 (Iba-1) rat prefrontal cortex (PFC); Western blot analysis applied respectively investigate Iba-1, inducible nitric oxide synthase (iNOS), arginase-1 (ARG1), inflammatory cytokines interleukin-1? (IL-1?), tumor necrosis factor-? (TNF-?) expression; The iNOS, ARG1, IL-1?, TNF-? sera rats detected enzyme-linked immunosorbent assay. We found induced impairment assessed morris test, up-regulated IL-1? inflammasome microglia, as indicated increased activation Iba-1 reduced level ARG1 PFC. conclude function after inhaling likely associated with M1/M2 polarization which triggered sevoflurane.